Challenges in DNA motion control and sequence readout using nanopore devices.
نویسندگان
چکیده
Nanopores are being hailed as a potential next-generation DNA sequencer that could provide cheap, high-throughput DNA analysis. In this review we present a detailed summary of the various sensing techniques being investigated for use in DNA sequencing and mapping applications. A crucial impasse to the success of nanopores as a reliable DNA analysis tool is the fast and stochastic nature of DNA translocation. We discuss the incorporation of biological motors to step DNA through a pore base-by-base, as well as the many experimental modifications attempted for the purpose of slowing and controlling DNA transport.
منابع مشابه
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ورودعنوان ژورنال:
- Nanotechnology
دوره 26 7 شماره
صفحات -
تاریخ انتشار 2015